OPERATION OF MICROPIPETTE

• OBJECTIVE:

• To lay down the procedure for operation of Micropipette.

• SCOPE:

This SOP is applicable to the procedure for Operation of Micropipette at {Company Name} {Location}.

• RESPONSIBILITY:
• Officer/Executive/Designee Quality Control – Shall be responsible for operation as per SOP.
• Head/Designee Quality Control – Shall be responsible for ensuring compliance as per SOP.
• ACCOUNTABILITY:

QA Head shall be Accountable for implementation of SOP.

• PROCEDURE:
  • Operating Procedure:
    • Set the required volume to be pipetted by using the setting ring.
    • It is advisable to carry out volume setting from the higher down to the lower value. i.e. first go above the desired volume and then return to the lower value.
    • The liquid which is to be aspirated is taken into a suitable vessel.
    • Attach suitable tips to the pipette firmly.
    • Press the control button to the first stop, immerse the pipette tip into the liquid.
    • Allow the control button to slide back slowly.
    • Before removing the tip wait approximately for three seconds, pull the tip out of liquid slowly.
    • Hold the tip in the suitable vessel and press down the control button slowly to the first Stop (measuring stroke) and wait until the liquid stops flowing.
    • Press down the control button to the second stroke (blow out) until the tip is completely empty.
    • Allow the control button to slide back slowly.
    • Tips are rejected by pressing the ejection button on the side of pipette.

NOTE:

• All the tips should be pre-wetted by aspirating and dispensing liquid two to three times before pipetting.
• Do not lay down the pipette when a filled pipette tip is attached as this may result in liquid entering the pipette.
• REFERENCES:

Not Applicable

• ANNEXURES: 

Not Applicable

        ENCLOSURES: SOP Training Record.

•  DISTRIBUTION:
• Controlled Copy No. 01  :          Head Quality Assurance
• Controlled Copy No. 02  :         Head Quality Control
• Master Copy                    :          Quality Assurance Department
• ABBREVIATIONS:

• REVISION HISTORY:             

CHANGE HISTORY LOG