OPERATION OF LINOMAT 5 TLC SPOTTER MAKE: CAMAG

• PROCEDURE:
• MAKE: CAMAG
• Preliminary operational instructions for Linomat 5:
• Check that the cable, electrical connection and connection of gas line are proper before Switching on the instrument.
• Switch on the Linomat 5. Open the valve of the gas cylinder and adjust the pressure to 58-87 psi.
• Check the turret is at perpendicular to the stage (TLC plate table), move it if necessary.Switch on the main and instrument.
• Procedure of spotting on Linomat 5:
• Make sure that plate stage is in front position and the syringe lever arm is at the top of the turret.
• Carefully insert the syringe through the syringe holder into the spray ahead and lower the syringe carefully until it rests in the spray head.

Note: The volume of the syringe in use must be set in the user dialog. If a wrong syringe volume is entered, the application volumes will all be wrong.

• Insert a waste TLC plate beside the stage, just below the syringe position.
• Place the TLC plate on the stage in a proper position and secure it with magnetic strips.
• Create the new method using following parameters:
• Analysis: Select the sample application as ‘Linomat- 5’ and development as ‘Chamber’ and rest of the parameters will be as default ones.
• Stationary phase: Details like plate size, material name, manufacturer, Batch Number etc. will be entered.
• Sample application:
• Linomat 5 General: Application volume should be the syringe capacity
• Sequence: Select the syringe size (Either 100µl (or) 500µl), Number of tracks and band length.
• While creating the sequence if different application volumes are required apply the same and change the position of spotting if required using number of tracks if required.
• After entering all required fields save the method.
• After creation of method take the method printout before executing to next step.
• When the system is ready for spotting ‘Execute next step’ command has to be given.
• When the analysis is completed, switch off the power supply, turn off the valves of the gas supply, remove the TLC plate, and clean the sample dosage syringe.
• After using the instrument make the logbook entry as per format-I.

• ANNEXURES: 

Annexure-I
TLC USER LOGBOOK