CALIBRATION OF UV CABINET

• OBJECTIVE:

• To lay down the procedure for Calibration of UV- cabinet.

• SCOPE:

This SOP is applicable to the procedure for Calibration of UV- cabinet at {Company Name} {Location}.

• RESPONSIBILITY:
• Executive/Designee Quality Control is responsible to calibrate the instrument as per SOP.
• Head QC: Ensure the compliance of SOP.
• ACCOUNTABILITY:

             QA Head shall be Accountable for implementation of SOP.

• PROCEDURE:
  • Calibration Procedure:
    • Preparation of Alcohol (Ethanol 96%): Dilute 48 ml of Ethanol absolute (Not less than 99.5%) to 50 ml with purified water.
    • Weigh accurately about 10mg of Sodium salicylate into a 25 ml volumetric flask, add 10 ml Ethanol (96 %) to dissolve and dilute the volume with the same (0.04%).
    • Apply 5 ml of the above solution to Silica gel G coated plate.
    • Place plate in cabinet and switch on the short wavelength (254 nm) and examine the spot in a position normal to the radiation.
    • At the maximum intensity at about 254 nm, the standard spot of Sodium salicylate with a diameter of about 5 mm on chromatographic plate shall be revealed.
    • Weigh accurately about 20 mg of Sodium salicylate in 10 ml volumetric flask, add 5 ml of ethanol (96%) to dissolve and dilute the volume with the same (0.2%).
    • Apply 5 ml of the above solution to Silica gel G coated plate.
    • Place plate in cabinet and switch on the long wavelength (365 nm) and examine the spot in a position normal to the radiation.
    • At the maximum intensity at about 365 nm, the standard spot of Sodium salicylate with a diameter of about 5mm on chromatographic plate shall be revealed.
    • Enter the results in calibration record as per Format-I.
    • Frequency: Quarterly.
• REFERENCES:

British Pharmacopeia

European Pharmacopeia

• ANNEXURES: 

        ENCLOSURES: SOP Training Record.

•  DISTRIBUTION:
• Controlled Copy No. 01  :          Head Quality Assurance
• Controlled Copy No. 02  :         Head Quality Control
• Master Copy                    :          Quality Assurance Department
• ABBREVIATIONS:

• REVISION HISTORY:             

CHANGE HISTORY LOG

Annexure-I
CALIBRATION RECORD FOR UV CABINET