CLEANING OF GLASSWARE USED IN CHEMICAL AND INSTRUMENTATION ANALYSIS

• OBJECTIVE:

To lay down the procedure for cleaning of Glassware used for analytical purposes.

• SCOPE:

This SOP is applicable for the procedure for cleaning of Glassware used for analytical purposes at {Company /name} {Location}.

• RESPONSIBILITY:

• Officer/Executive/Designee Quality Control – Shall be responsible for cleaning of glassware as per SOP.
• Head/Designee Quality Control – Shall be responsible for ensuring compliance as per SOP.

• ACCOUNTABILITY:

QA Head shall be Accountable for implementation of SOP.

• PROCEDURE:

• After completion of analytical work, keep all the glassware in tray and transfer it into washing area.  
  • General glassware:
    • Wear a rubber apron, safety goggles, and rubber hand gloves.
    • Empty the glassware under the running tap water.
    • Remove any external marking on glassware with solvent.
    • Clean the glassware with suitable nylon brush by using cleaning solution “Teepol solution or Equivalent”.
    • Clean oily and greasy content by using 70% nitric acid solution.
    • Wash the glassware with tap water up to foaming not observed in water by vigorous shaking.
    • Rinse the glassware with purified water.
    • Ensure complete removal of cleaning solution by visual checking.
    • Keep the glassware in inverted position for water drain out from glassware.
    • Finally, the Glassware shall be rinsed with acetone and dried at 40°C.
    • After drying the Glassware shall be removed and stored in their designated places.
    • Ensure cleaning of glassware by visual inspection before use for analysis.
  • Pipette washing:
    • Rinse used pipette with tap water by tubing connected to water line.
    • Rinse it 2-3 times in plastic cylinder having cleaning solution. “Teepol or equivalent”.
    • Rinse it with tap water by tubing connected to water line up to foaming not observed in pipette.
    • Finally rinse the pipette with purified water.
    • Ensure complete removal of cleaning solution from pipette by visual checking.
    • Keep clean pipette in pipette stand for water drain out.
    • Finally, the Glassware shall be rinsed with acetone and dried at 40°C.
    • Transfer clean and dry pipettes in their designated places.
    • Ensure cleaning of pipette by visual inspection before use for analysis.
  • Burette washing:
    • Rinse used burette with tap water by tubing connected to waterline.
    • Rinse it with tap water by tubing connected to waterline up to foaming not observed in Burette.
    • Finally rinse the burette with purified water.
    • Ensure complete removal of cleaning solution from burette by visual checking.
    • Keep clean burette in burette stand for water drain out.
    • Dry it as per the general procedure mentioned above.
    • Transfer clean and dry burette in their designated places.

• REFERENCES:

Not Applicable

• ANNEXURES: 

Not Applicable

      ENCLOSURES: SOP Training Record.

• DISTRIBUTION:

• Controlled Copy No. 01  :          Head Quality Assurance
• Controlled Copy No. 02  :         Head Quality Control
• Master Copy                    :          Quality Assurance Department

• ABBREVIATIONS:

• REVISION HISTORY:              

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