GROWTH PROMOTION TEST

• PROCEDURE FOR THE GROWTH PROMOTION TEST:

• A growth promotion test is a quality control procedure used in microbiology. It is a relatively simple test, where the medium is challenged with a small number of microorganisms to assure its nutritive properties and to demonstrate that it enables organism growth.
• Each dehydrated / ready prepared Media, which is used for microbiological testing should be subjected for Growth Promotion test.
• Each lot of received medium should be tested for growth promotion as per GTP.Assign GPT Reference Number to each media undergoing for Growth Promotion Test.
• Assigning GPT Reference Number to Media:
• All the media undergoing Growth promotion test shall be identified with unique numbering system.
• The numbering system consists of 14 characters.
• First three characters “GPT” denote Growth Promotion Test.
• Next character is a “/” slash.
• Next three characters denote Name of the Media undergoing GPT.
• Next character is a “/” slash.
• Next three characters are the serial number starting from 001 in every year.
• Next character is a “/” slash.
• Next two characters “25” denote year 2025.
• Example: The first Soyabean Casein digest Agar medium undergoing growth promotion test in the year of 2022 shall be numbered as GPT/SCD/001/25.
• Procedure for Growth promotion Test:
• Perform the Growth promotion test as per the GTP.
• Record the results of growth promotion in Format.
• In case of failure in GPT for the media, following action shall be taken:
• If GPT fails fewer than two organisms, re-inoculation with the fresh culture shall be carried out and investigation shall includes Conditions of culture preparation & storage, Conditions of media preparation and Conditions of media sterilization.
• For any batch of agar that fails to meet GPT criteria on first test and on re-inoculation PNC shall be initiated and investigation shall be performed. During investigation review (but not limited to)- conditions of culture preparation & storage, Conditions of media preparation, Conditions of media sterilization, Other possibly related GPT Incidents and Data obtained in testing using the media in question.
• Investigate the GPT failure as per Format-II.
• Based on the investigation necessary corrective and preventive action (CAPA) shall be taken.
• If the media shows satisfactory growth then the media shall be used for routine analysis.

• ANNEXURES: 

• ABBREVIATIONS:

Annexure-I
GROWTH PROMOTION TEST REFERENCE NUMBER ALLOCATION REGISTER

Annexure-II
GROWTH PROMOTION TEST FAILURE INVESTIGATION REPORT