Wear gloves, nose masks and safety goggles before starting the activity.
Transfer the required quantity of original disinfectant in a washed and dried glass beaker.
Collect the required quantity of purified water in a sterilized/sanitized container.
Mix the original disinfectant with purified water to obtain the specified concentration of disinfectant solution as mentioned in the following table.(For One liter solution )
S. No
Name of Disinfectant
Concentration
Volume of Disinfectant (ml)
Volume of Purified water (ml)
1
Microbac forte
2 %
20
980
2
Bacillocid special
2 %
20
980
3
Korsolex, Rapid
2 %
20
980
4
Isopropyl Alcohol
70 %
700
300
Arrange the filtration assembly with 0.2 micron membrane filter.
Filter the disinfectant by passing through the membrane filter and collect it in a clean, dried and Sterilized/sanitized container.
Transfer the required quantity of filtered disinfectant solution in a stainless steel container which has been Sterilized/sanitized previously.
Use the filtered disinfectant solution for routine cleaning and sanitization of Microbiology Laboratory on weekly rotation basis.
Enter the details of preparation in Disinfectant solution preparation record (Format-I).
Frequency: Once in a week or whenever required.
ANNEXURES:
ANNEXURE NO.
TITLE OF ANNEXURE
Annexure-I
Disinfectant solution preparation record
ABBREVIATIONS:
No.
:
Number
SOP
:
Standard Operating Procedure
ml
:
Milli liter
%
:
Percentage
QC
:
Quality Control
Annexure-I DISINFECTANT SOLUTION PREPARATION RECORD
