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CLEANING AND STERILIZATION OF GLASSWARE USED IN MICROBIOLOGY

  • PROCEDURE:
  • CLEANING OF GLASS WARE:
  • Wash the glassware first with potable water and then with the soap solution.
  • Wash the glassware in running water until free from the traces of soap solution.
  • Finally rinse the glassware with purified water.
  • Dry the glassware in Hot Air Oven at 60°C for one hour.
  • STERILIZATIONOF GLASS WARE:
  • Wrap the mouth of glassware such as conical flask and bottles with Kraft paper/Crepe Paper.
  • Materials like Petri dishes and pipettes shall be wrapped in Kraft paper/Crepe Paper.
  • Stick the chemical indicator tape on the glassware to be sterilized.
  • Sterilize the glassware in autoclave and operate the autoclave as per the SOP.
  • Sterilize the glassware as per the validated cycle.
  • Label the sterilized articles as per Format-I.
  • Sterilized glassware shall be used within 72 hrs., if not used within the specified time, shall be re- sterilized before use.
  • ANNEXURES: 
ANNEXURE NO.TITLE OF ANNEXURE
Annexure-ISterilization Label
  • ABBREVIATIONS:
No.:Number
SOP:Standard Operating Procedure
IPA:Iso Propyl alcohol
QC:Quality Control

Annexure-I
STERILIZATION LABEL

STERILIZATION LABEL
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