- PROCEDURE:
- Wear gloves, nose masks and safety goggles before starting the activity.
- Transfer the required quantity of original disinfectant in a washed and dried glass beaker.
- Collect the required quantity of purified water in a sterilized/sanitized container.
- Mix the original disinfectant with purified water to obtain the specified concentration of disinfectant solution as mentioned in the following table.(For One liter solution )
| S. No | Name of Disinfectant | Concentration | Volume of Disinfectant (ml) | Volume of Purified water (ml) |
| 1 | Microbac forte | 2 % | 20 | 980 |
| 2 | Bacillocid special | 2 % | 20 | 980 |
| 3 | Korsolex, Rapid | 2 % | 20 | 980 |
| 4 | Isopropyl Alcohol | 70 % | 700 | 300 |
- Arrange the filtration assembly with 0.2 micron membrane filter.
- Filter the disinfectant by passing through the membrane filter and collect it in a clean, dried and Sterilized/sanitized container.
- Transfer the required quantity of filtered disinfectant solution in a stainless steel container which has been Sterilized/sanitized previously.
- Use the filtered disinfectant solution for routine cleaning and sanitization of Microbiology Laboratory on weekly rotation basis.
- Enter the details of preparation in Disinfectant solution preparation record (Format-I).
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Frequency: Once in a week or whenever required.
- ANNEXURES:
| ANNEXURE NO. | TITLE OF ANNEXURE |
| Annexure-I | Disinfectant solution preparation record |
- ABBREVIATIONS:
| No. | : | Number |
| SOP | : | Standard Operating Procedure |
| ml | : | Milli liter |
| % | : | Percentage |
| QC | : | Quality Control |
Annexure-I
DISINFECTANT SOLUTION PREPARATION RECORD
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