PROCEDURE FOR CALIBRATION OF POLARIMETER

• PROCEDURE:
• Make – Rudolph Research Analytical
• Operate instrument as per operation SOP.
• Sample compartment shall be kept empty while switching on instrument.
• Switch on the instrument by using the ON/OFF switch present on backside of the instrument.
• Keep the instrument ON condition at least for10 minutes before operation and wait until the reading on the display come to stable position.
• Press the Zero key and wait until the display shows 0.00 reading.
• Temp Trol Quartz control plate shall be placed right side in the trough.
• Insert the built in temperature probe into the Temp Trol Quartz control plate.
• Set the temperature to 20°C and allow it to stabilize.

• Measure the optical rotation of the Quartz control plate at wavelength mentioned below.

Note: To the above acceptance limits add instrument Accuracy ± 0.01.

• Temperature Calibration:
• Select temperature calibration by pressing the temperature calibration key.
• Place the temperature validation cell into the trough on to the right hand side of the trough.
• Be sure the foot of Temp trol cell is clean and sitting flat against the clean temp trol temperature transmission surface to allow for maximum heating and cooling efficiency.
• The built in temperature probe shall be placed in the temperature probe well of the temperature validation cell and tighten the finger nut to a secure.
• Fill the temperature validation cell with water by adding the water into the temperature validation cell.
• Fill the cell until the water level is just above the copper sleeve inside the thermometer well.
• Insert a calibrated thermometer into the thermometer well of the temperature validation cell.
• Set the temperature to 20°C and allow the built in probe temperature reading to stabilize at 20°C ± 0.1°C for 10 minutes and note down the temperature of the calibrated thermometer.
• The same procedure shall be followed for 25°C and 30°C respectively and note down the reading.

• Acceptance criteria shall be ± 0.2°C
• Enter the details of the usage in the User logbook.

• ANNEXURES: 

Annexure-I
CALIBRATION RECORD OF POLARIMETER